Isabelle of Portugal: Isabel de Aviz, Infanta de Portugal was born on 4 October 1503 at Lisbon, Portugal. She was the daughter of Manuel I de Aviz, Rei de Portugal and Maria de Castilla y Aragón, Infanta de Castilla.She married Karl V von Habsburg, Holy Roman Emperor, son of Felipe I von Habsburg, Rey de Castilla and Juana, Reina Juana de Castilla, on 11 March 1526 at Seville, Spain. She died on 1 May 1539 at age 35 at Toledo, Spain.
Maria Anna of Neuberg, Queen of Spain as consort of Carlos II. 1667-1740. Pretty but difficult, she was chosen as queen because the king had no issue, and her parents had had 17 children. Drove everyone bats (at a guess, she suffered from some sort of congenital mental instability) and ironically, had no issue. Lots of inbreeding in her family--related to Anne of Cleves in 2 lines, and to Hesse in at least 2.