Molecular biology

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8.7: DNA Analysis- Blotting and Hybridization - Biology LibreTexts Microbiology, Biotechnology, Biochemistry, Molecular Genetics, Cell Biology, Immunology, Molecular Biology, Medical Knowledge, Biochemistry Notes

Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.