Thin layer chromatography is an effective procedure that helps in separating different chemicals inside a mixture. This process can help in separating all kinds of non-volatile chemicals. http://www.informationbible.com/rf-values-in-thin-layer-chromatography-574753.html
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Thin layer chromatography (TLC) is very useful to chemists as an analytical technique to separate and identify the compounds in a mixture. Only very small samples of the analyte are needed much less than a milligram.