Column chromatography for beginners. First, look after an immobile phase (usually silica or alumina) what was tested before with the correct eluent (mixture of 2 or more organic solvent). If the mixture of compounds separate with this mixture on a TLC (thin layer chromatography) what is made from the same immobile phase it will hopefully work on a larger scale also.
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Thin Layer Chromatography In Chiral Separations And Analysis PDF
This page is an introduction to chromatography using thin layer chromatography as an example. Although if you are a beginner you may be more familiar with paper chromatography, thin layer chromatography is equally easy to describe and more straightforward to explain.
Thin layer chromatography (TLC) is very useful to chemists as an analytical technique to separate and identify the compounds in a mixture. Only very small samples of the analyte are needed much less than a milligram.