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EMBL Practical Course Next Generation Sequencing: Total RNA sequencing library preparation and validation EMBL Heidelberg, Germany Wedne...

Small RNA Sequencing Small RNA has been to important regulators in recent years and has significant functions in many cellular processes including development... http://www.cd-genomics.com/Small-RNA.html

Informatics for RNA-sequence Analysis (QC) (2014) | Bioinformatics.ca

RNA sequencing of 750-year-old barley virus sheds new light on the Crusades

says while the photo doesn't match the story -please read about this research re reducing the Johne's disease threat with chlorine and stainless steel -- ScienceDaily

New microRNA Analysis App in BaseSpace - miRNAs are a class of non-coding RNAs mainly involved in post-transcription control of gene expression although they are also involved in other non-canonical functions. Furthermore, they represents an interesting class of biomarkers for a large variety of diseases. Small RNA-sequencing is consolidating as the election method for the quantification of miRNAs. The steps needed to analyze Small RNA-sequencing data require the use of dedicated hardware…

RNA Sequencing Helps Unravel Disease Pathology In Atopic Dermatitis | MedicalResearch.com Medical Research Interviews and NewsMedicalResearch.com Medical Research Interviews and News

RNA-sequencing (RNA-seq) is a powerful technique for the identification of genetic variants that affect gene-expression levels, either through expression quantitative trait locus (eQTL) mapping or through allele-specific expression (ASE) analysis. Given increasing numbers of RNA-seq samples in the public domain, researchers from the University of Groningen studied to what extent eQTLs and ASE effects can be identified when using public RNA-seq data while deriving the genotypes from the…

Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud

FunPat – function-based pattern analysis on RNA-seq time series data - Dynamic expression data, nowadays obtained using high-throughput RNA sequencing, are essential to monitor transient gene expression changes and to study the dynamics of their transcriptional activity in the cell or response to stimuli. Several methods for data selection, clustering and functional analysis are available; however, these...

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RNA-sequencing (RNA-Seq) technologies hold enormous promise for novel discoveries in genomics and transcriptomics. In the past year, a surge of reports has analyzed RNA-Seq data to gain a global view of the RNA editome. Opposing results have been presented, giving rise to extensive debate surrounding one of the first such studies in which a daunting list of all 12 types of RNA-DNA differences (RDDs) were identified. Although a consensus is forming that some of the initial “paradigm-shifting”…

OncoRep – an n-of-1 reporting tool to support genome-guided treatment for breast cancer patients using RNA-sequencing - Breast cancer comprises multiple tumor entities associated with different biological features and clinical behaviors, making individualized medicine a powerful tool to bring the right drug to the right patient. Next generation sequencing of RNA (RNA-Seq) is a suitable method to detect targets for individualized treatment. Challenges that arise are: i) preprocessing and…

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CRISPR-CAS9 gene editing complex from Streptococcus pyogenes. The Cas9 nuclease protein uses a guide RNA sequence to cut DNA at a complementary site.

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RNA-Seq reveals distinct features of exosomal small RNA - Functional biomolecules, including small noncoding RNAs (ncRNAs), are released and transmitted between mammalian cells via extracellular vesicles (EVs), including endosome-derived exosomes. The small RNA composition in cells differs from exosomes, but underlying mechanisms have not been established. Researchers from the VU University Medical Center generated small RNA profiles by RNA sequencing...

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Translate is a tool which allows the translation of a nucleotide (DNA/RNA) sequence to a protein sequence.

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Capture RNA-seq for degraded and clinical RNA - To facilitate the use of RNA sequencing beyond cell lines and in the clinical setting, researchers at the University of Michigan developed an exome-capture transcriptome protocol with greatly improved performance on degraded RNA. Capture transcriptome libraries enable measuring absolute and differential gene expression, calling genetic variants, and detecting gene fusions. Through validation against gold-standard poly(A) and Ribo-Zero…

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Evaluating the Stability of RNA-Seq Transcriptome Profiles in Whole Blood Over Time - Researchers at the FDA have developed methods to evaluate the stability of rat whole blood expression obtained from RNA sequencing (RNA-seq) and assess changes in whole blood transcriptome profiles in experiments replicated over time. Expression was measured in globin-depleted RNA extracted from the whole blood of Sprague-Dawley rats, given either saline (control) or neurotoxic doses of amphetamine…

Defining cell lineages by single-cell RNA-Seq - Researchers at the Francis Crick Institute provide fundamental insights into early human development by single-cell RNA-sequencing of human and mouse preimplantation embryos. They elucidate conserved transcriptional programs along with those that are human-specific and validate their RNA-sequencing findings at the protein level. They identified several genes exclusively expressed in the human pluripotent epiblast including the transcription…

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Genomic profiling of lesional and nonlesional skin of patients with atopic dermatitis (AD) using microarrays has led to increased understanding of AD and identification of novel therapeutic targets. However, the limitations of microarrays might decrease detection of AD genes. These limitations might be lessened with next-generation RNA sequencing (RNA-seq). RNA-seq and microarrays were performed to identify differentially expressed genes in lesional versus nonlesional skin from 18 patients…

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The benefits and applications of immune repertoire sequencing services

Next generation sequencing services allows immune repertoire sequencing to amplify & sequence the immune repertoire. T cell & B cell repertoire sequencing of immune repertoire yields high quality throughput sequencing.

Researchers unlock another piece of the RNA sequencing puzzle.

Now, a study from researchers at Rockefeller University and Columbia University has identified a protein that recognizes a chemical instruction tag affixed to an RNA sequence, an important step in the decision-making process. The team state that their findings help to explain how the destiny of an RNA sequence is achieved. The opensource study is published in the journal Cell.

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CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats, is a central part of the bacterial immune system and handles sequence recognition. Cas9 – Cas stands for CRISPR-assisted – is an RNA-guided enzyme that handles the snipping of DNA strands at the specified sequence site. Together, CRISPR and Cas9 can be used to precisely edit the DNA instructions in a targeted genome for making desired types of proteins.