Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary diseases. It is used for the analysis of unique patterns in DNA fragments in order to genetically differentiate between organisms – these patterns are called Variable Number of Tandem Repeats (VNTRs).
The hit CBS television series CSI: Crime Scene Investigators achieves much of its acclaim from popularizing the state-of-the-art forensics techniques analysts use to catch criminals and acquit the innocent.
Amplified Fragment Length Polymorphism PCR _ Digestion of total cellular DNA with restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments. Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences. Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern.