Does removal of secondary metabolites improve Zingiberaceous nuclei quality
The family Zingiberaceae is well known for its range of secondary constituents and bioactive metabolites such as flavonoids phenolic acids and essential oils known to increase nuclei stickiness in cell free lysate. Photomicrographs obtained from bio-SEM analyses of A. zerumbet shoot nuclear suspensions using different isolation buffers. (A) Arumuganathan and Earle buffer (B) Galbraiths buffer (C) General purpose buffer (D) LB01 buffer (E) Maries nuclear isolation buffer (F) MB01 buffer (G)…
Using a live/dead to remove dead cells can improve your staining. (a). Use of forward and side scatter gating (red rectangle) may not remove all dead cells and some non-specific binding may still be present. (b). Exclusion of dead cells using propidium iodide staining (red rectangle) means less non-specific binding and easier identification of positively stained populations. Images shown here are human peripheral blood stained with CD14 (MCA1568) and CD3 (MCA463).
Green fluorescent protein (GFP)-expressing and red fluorescent protein (RFP)-expressing Sinorhizobium meliloti attached to root hairs of transgenic Medicago truncatula expressing GFP::talin, which marks the actin cytoskeleton.