DNA Polymerase: functions to polymerize newly forming strands of complementary DNA in the 5 to 3 prime direction, using an existing strand of DNA as a template; may also function to proofread for errors in the new DNA strand
Transcription proceeds in the following general steps: One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA. RNA polymerase creates a transcription bubble, which separates the two strands of the DNA helix. This is done by breaking the hydrogen bonds between complementary DNA nucleotides. RNA polymerase adds matching RNA nucleotides to the complementary nucleotides of one DNA strand.
Central Dogma of Molecular Biology -DNA makes RNA -RNA makes Proteins -Proteins help DNA (and sometimes RNA) to replicate itself -And some Proteins, like those from retrovirus HIV can turn RNA back into DNA (called complementary DNA or cDNA for short)
FISHing in the Genomic Testing Age ! Genetic analysis has come a long way; we now have an ever-expanding collection of analytical tools in the diagnostic laboratory. So why do we still need a technique that usually only looks at one or two loci? The simple answer is that results from fluorescence in-situ hybridization (FISH) can quickly confirm diagnoses guide clinicians judgements regarding differential diagnoses and correlate results with clinical riskthus enabling an informed choice of…
The largest brain study of its kind had found a gene linked to intelligence. Searching for a genetic explanation for brain disease, the scientists stumbled upon a minute variant in a gene called among people who had larger brains and scored highe
Real-time (RT) reverse-transcription polymerase chain reaction (PCR) is currently the most sensitive and reliable technique for detecting viruses. The technology involves making complementary DNA copies of viral RNA, multiplying the number of DNA copies and staining them with a fluorescent dye. The subtle differences in fluorescence intensity reflect the presence and quantity of viral RNA. Chapter 96
The picture shows a microscope image of micrometer-sized flowers self-assembled from silica particles equipped with sticky “DNA-glue". The core of the flowers are 1 micrometer-sized silica particles functionalized with a DNA strand with a green dye. The petals of the flowers consist of 2 micrometer-sized particles, containing the complementary DNA strand with a red dye. Using these particles in different concentrations, beautiful flower-like structures form due to the DNA strands.